Flow Cytometry and Immune Monitoring Shared Resource Facility

FCIM SRF Overview

The overall mission of the Flow Cytometry and Immune Monitoring Shared Resource Facility (FCIM SRF) is to provide technical and scientific support for MCC investigators whose research programs require flow cytometric cell analysis and/or sorting or other immune-based cell assays. FCIM provides access to a team of expert immunologists and state of art instruments to aid pre-clinical, translational, and clinical research activities at the Markey Cancer Center. The FCIM team comprises experts in flow cytometry, tumor immunology, immunotherapy, vaccine biology, inflammation, and immune biomarker discovery,. We provide both fee-based services and scientific collaboration for research requiring immunology expertise for both human or animal studies.

Visit the full Flow Cytometry and Immune Monitoring Shared Resource Facility site for more information.

Full FCIM Website

Specific Aims

  1. Provide cost-effective state-of-the-art flow cytometry and cell sorting services for basic, preclinical, translational, and clinical researchers.
  2. Provide cost-effective state-of-the-art immune monitoring services for basic, pre-clinical, translational, and clinical researchers.
  3. Provide educational training and mentoring to MCC members and/or laboratory staff in proper use of self-service instrumentation and appropriate experimental design and data analysis.
  • Flow cytometric analysis 
    • Multi-parameter Cell phenotyping including both traditional and spectral flow cytometry 
    • DNA content and cell cycle
    • Apoptosis and necrosis
    • Cytotoxicity assays
    • Oxidative stress
    • Intracellular cytokine analyses
    • Signal transduction 
    • Cell activation
  • Cell sorting
    • Sterile cell sorting
    • Single cell sorting
  • Magnetic cell sorting
    • Positive cell isolation
    • Negative cell isolation
    • Rare cell enrichment
  • Immune monitoring
    • Peripheral blood cell isolation
    • Plasma/serum isloation
    • Cell banking services
    • Cytokine/growth factor analysis 
    • ELISA 
    • Elispot 
    • Luminex 
    • Leukocyte functional assays 
    • Proliferation 
    • Cytokine synthesis 
    • Antibody synthesis 
    • Cell cytotoxicity 
    • Cellular product services 
    • T cell expansion 
    • Macrophage/DC generation 
    • Antibody titers 
    • Immune Assay Design Services 
    • Research collaborations 
    • Isoplexis analysis

 

Requests for services can be made by contacting either the Flow Cytometry & Cell Sorting Lab or the Immune Monitoring Lab.

Reservations for services on particular FCIM instrumentation can be made through the PPMS website.
 

jamie sturgill
Jamie Sturgill, PhD

FCIM SRF Director

siva gandhapudi
Siva Gandhapudi, PhD

FCIM SRF Assistant Director

Hong Jiang, MD, PhD
Hong Jiang, MD, PhD
FCIM Manager

NOT PICTURED

  • Cuiping Zhang, Flow Cytometry Technician
  • Rita Avdiushko,Immune Monitoring Technician

Flow Cytometry and Cell Sorting Lab: 
Lab Suite 076, Healthy Kentucky Research Building 
Telephone: 859-323-6859 
Email: FCIMCore@uky.edu

Immune Monitoring Lab: 
Lab Suite 076A, Healthy Kentucky Research Building 
Telephone: 859-323-5584
Email: FCIMCore@uky.edu

Visit FCIM main website for facility brochure and more information.

Prices and lab policies for services can be found here

Where is the FCIM located?

The core is located in two adjoining labs, Lab Suite 076 (Flow Cytometry) and Lab Suite 076A (Immune Monitoring) in the Healthy Kentucky Research Building (HKRB).

What are the FCIM hours?

Staff are at the facility Monday through Friday, 9:30 am - 5:00 pm. 

Who should I contact regarding scheduling and general questions about services and billing for Flow Cytometry?

Please contact Jamie Sturgill by email or by calling 859-562-0124.

Who should I contact regarding scheduling and general questions about services and billing for Immune Monitoring?

Please contact Siva Gandhapudi, PhD by email or at 859-323-5584

Who should I contact concerning experimental design, assay development, and biosafety issues?

Please contact facility director, Jamie Sturgill, Ph.D., 859-562-0124, or assistant director Siva Gandhapudi, PhD by email or at 859-323-5584. You may also email FCIMCore@uky.edu for assistance.

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Acknowledge the FCIM SRF

Investigators are required to acknowledge the Markey Cancer Center Flow Cytometry and Immune Monitoring Shared Resource Facility (FCIM SRF) in any publications that result from the use of biospecimens or information received through the MCC FCIM SRF. Please use the following statement to acknowledge the FCIM SRF.

“This research was supported by the Flow Cytometry and Immune Monitoring Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558).”

NCI Comprehensive Cancer Center - A Cancer Center Designated by the National Cancer Institute

Markey Cancer Center is designated by the National Cancer Institute as a Comprehensive Cancer Center – a distinction that recognizes our commitment to accelerating precision cancer research and care to patients. We are the first and only NCI-Comprehensive Cancer Center in Kentucky, and one of 57 in the nation.

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