The Genomics Core Laboratory, a College of American Pathologists-accredited facility, was established in 2015 as part of the UK HealthCare Department of Pathology & Laboratory Medicine and Markey Cancer Center genomics initiative.
Our facility occupies 4,500 square feet within the UK HealthCare Clinical Laboratories. The overall mission of our laboratory is to accelerate the clinical genomics, precision medicine and genomic research interests at the University of Kentucky by providing cutting-edge genomic technology services. This facility also functions as Markey Cancer Center’s Oncogenomics Shared Resource Facility.
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Equipment Available
Our laboratory is equipped with:
- HiSeq 2500
- MiSeq
- Capillary sequencers
- Pyrosequencer
- Affymetrix Microarray
- NanoString
- Real-time PCR
- Luminex analyzer
- Covaris ultrasonicator
- Bioanalyzers
We have several laboratory automation systems for sample preparation and assay setup. In addition, this center is equipped with an isolated high-speed network connection (100 GB) for fast data transfer to the secured genomic storage facility within the campus and for computational analysis.
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Wide Range of Clinical Services
Our center provides a wide range of genomic services for clinical care, including:
- Chromosomal microarray.
- Genotyping for pharmacogenetics.
- DNA sequencing for cancer and genetic disorders.
- Capillary.
- Next-generation.
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Research Genomics Services
We Provide Investigators Access to a Wide Range of Genomic Services
Next-Generation Sequencing
DNA Sequencing
- Whole genome sequencing
- Human and other organism
- We prepare NGS libraries
- Targeted resequencing
- Amplicon sequencing and small gene panels
- Investigators provide the probes/amplicons/libraries
- Whole exome sequencing
- We prepare the libraries
- Agilent Clinical Research Exome
- Roche Nimblegen MedExome
- Illumina Rapid Exome
RNA Sequencing
- Whole transcriptome sequencing (RNA-seq)
- We prepare NGS libraries/investigators can also prepare libraries
- Targeted RNA-sequencing
- Investigators provide the NGS libraries
- Methylation sequencing
- Whole genome methylation sequencing
- Investigators can provide NGS libraries
- Chip-sequencing
- NGS library preparation & sequencing only
- Investigators perform IP and provides the captured fragments
Sanger Sequencing
- Tier I – Sequencing only
- Investigators perform PCR, sequencing reaction and cleanup
- Tier II – Clean up and sequencing
- Investigator perform PCR and sequencing reaction
- Tier III – Sanger sequencing reaction, cleanup and sequencing
- Investigator perform PCR
Fragment Analysis
- Client performs reactions and fragment analysis on the ABI 3730
Gene Expression Microarray
- All standard arrays
- miRNA array
- Custom arrays
Other Services
- NanoString
- Real-time-PCR
- Bioanalysis
- Covaris ultrasonication for ChIP-sequencing
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Locations
Genomics
UK HealthCare Genomics Core Laboratory
Room HA629UK Albert B. Chandler Hospital - Pavilion HA
800 Rose St.
Lexington KY 40536Call 859-323-5327Call 859-323-5984Shipping address
UKHC Genomics Core Laboratory
800 Rose St., HA629
Lexington KY 40536-0084
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