Immunohistochemistry and in situ Hybridization
Immunohistochemistry (IHC)
- Demonstrates protein in tissue by reaction of antibody raised against that protein, generally followed by amplification and visualization with a chromogen.
- BPTP can provide select antibodies—inquire about availability and cost: markey.bstp@uky.edu
- BPTP can optimize your antibody—please contact us before ordering: markey.bstp@uky.edu
- Fluorescent detection is available.
- Multi-plex staining (IHC or IF) is available, pending protocol optimization.
In situ Hybridization (iSH)
- Demonstrates RNA transcripts in tissue by hybridiziation of RNA probe against that transcript, followed by amplification and visualization with a chromogen.
- BPTP utilizes ACD RNAScope assays for the Ventana Discovery Ultra—https://acdbio.com/automated-assay-roche
- User must provide probe of interest and an appropriate positive AND negative probe. BPTP supplies all other supplies.
- BPTP will optimize the procedure on your tissue using positive and negative probes.
- Dual-plex staining is available.
- Consultation is required before staring an iSH project or ordering reagents. Contact markey.bstp@uky.edu
