Next Generation Sequencing

Next-generation sequencing, also known as massively parallel sequencing, is the term used to describe the modern sequencing technology. Next-generation sequencing is capable to sequencing millions of copies of DNA fragments in a single reaction. 

UK HealthCare Genomics Core Laboratory has five Illumina® next-generation sequencing systems, the NovaSeq 6000, two NextSeq 2000, and two MiSeq benchtop sequencers. The Illumina sequencing systems utilize sequencing by synthesis (SBS) technology and support both single-read and paired-end libraries.
In addition to sequencing services, UK Genomics Core Laboratory offers select library preparation services.

Sample Submission Instructions

For inquiries or sample submission instructions, contact us at genomics@uky.edu. For directions, please see our About Us page. 

NGS Research Services Pricing, July 2022-June 2023

Sequencing Service Read Pairs (M) Output (Gb) UK Price* External Price*
NovaSeq S4 (300 cycles) 10,000 3000 $17,132  $          18,845.20
NovaSeq S4 (200 cycles) 10,000 2000 $15,393  $          16,932.30
NovaSeq S4 (35 cycles); 1x35 10,000 350 $12,543  $          13,797.30
NovaSeq S2 (300 cycles) 4,100 1250 $11,474  $          12,621.40
NovaSeq S2 (200 cycles) 4,100 833 $10,767  $          11,843.70
NovaSeq S2 (100 cycles) 4,100 417 $8,704  $             9,574.40
NovaSeq S1 (300 cycles) 1,600 500 $6,346  $             6,980.60
NovaSeq S1 (200 cycles) 1,600 333 $5,875  $             6,462.50
NovaSeq S1 (100 cycles) 1,600 167 $4,696  $             5,165.60
NovaSeq SP (500 cycles) 800 400 $5,109  $             5,619.90
NovaSeq SP (300 cycles) 800 250 $3,694  $             4,063.40
NovaSeq SP (200 cycles) 800 167 $3,400  $             3,740.00
NovaSeq SP (100 cycles) 800 80 $2,635  $             2,898.50
NextSeq 2000 P3 (300 Cycles) 1,200 360 $6,562  $             7,218.20
NextSeq 2000 P3 (200 Cycles) 1,200 240 $4,955  $             5,450.50
NextSeq 2000 P3 (100 Cycles) 1,200 120 $3,616  $             3,977.60
NextSeq 2000 P3 (50 Cycles); 1x50 1,200 60 $2,545  $             2,799.50
NextSeq 2000 P2 (300 Cycles) 400 120 $3,927  $             4,319.70
NextSeq 2000 P2 (200 Cycles) 400 80 $2,995  $             3,294.50
NextSeq 2000 P2 (100 Cycles) 400 40 $1,656  $             1,821.60
NextSeq 2000 P1 (300 Cycles) 100 30 $1,441  $             1,585.10
MiSeq (600 Cycles) (v3) 25 15 $2,051  $             2,256.10
MiSeq (150 Cycles) (v3) 25 3.8 $1,272  $             1,399.20
MiSeq (500 Cycles) (v2) 15 8.5 $1,608  $             1,768.80
MiSeq (300 Cycles) (v2) 15 5.1 $1,445  $             1,589.50
MiSeq (50 Cycles) (v2) 15 0.85 $1,160  $             1,276.00
MiSeq Micro (300 Cycles) (v2) 4 1.2 $693  $                  762.30
MiSeq Nano (500 Cycles) (v2) 1 0.5 $578  $                  635.80
MiSeq Nano (300 Cycles) (v2) 1 0.3 $505  $                  555.50

Contact us at genomics@uky.edu
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*Prices listed are non-binding and subject to change. List price includes loading/pool QC (TapeStation and Qubit).

†Maximum run output, based on Illumina advertised specifications and is not guaranteed. Output may vary with library type and quality, base diversity, and more.