The facilities described in this section are core cancer center facilities and are given direct support from the cancer center. The services offered by these facilities foster and facilitate advances in the prevention, early detection and management of cancer. These combined resources support a broad spectrum of molecular, cellular, animal, human and behavioral/epidemiology studies for advancement of the overall mission of the center.
Biospecimen Procurement and Translational Pathology Shared Resource Facility
The Biospecimen Procurement and Translational Pathology Shared Resource Facility (BPTP SRF) procures and distributes biospecimens and provides pathologic expertise and support to University of Kentucky cancer researchers to perform specialized histopathologic, immunohistochemical and molecular analyses. The goals of the facility are to provide formalin-fixed paraffin-embedded tissues, frozen tissue, blood and other specimens, and histopathological and immunohistochemistry expertise for the studies related to the aims of MCC-supported research projects. Visit the BPTP SRF webpages for more information.
Biostatistics and Bioinformatics Shared Resource Facility
The Biostatistics and Bioinformatics Shared Resource Facility (BB SRF) is a cancer center-managed shared resource that plays a key collaborative role in providing scientific and statistical input across the entire spectrum of cancer research being performed at the Markey Cancer Center (MCC). The mission of the BB SRF is to apply statistical principles to enhance conduct of scientific research through collaborative interactions with MCC members. To this end, our primary goal is to provide a comprehensive, centralized, and accessible biostatistics support infrastructure staffed with high-level statistical expertise to support all phases of cancer studies: development, implementation, and post-study analyses. For more information, visit the BB SRF website or email firstname.lastname@example.org.
Cancer Research Informatics Shared Resource Facility
The Cancer Research Informatics Shared Resource facility (CRI SRF) facilitates collaborative research among members of the Markey Cancer Center through the optimal application of informatics technologies and methods that maximize the accessibility and usability of data, information and knowledge. The primary goal of the CRI SRF is to provide comprehensive and centralized data acquisition and informatics support that is readily available to cancer center members.
Flow Cytometry and Cell Sorting Shared Resource Facility
Flow cytometry and immune monitoring services, together with immune assay design services, support the central goal of FCCS to provide Markey Cancer Center researchers the opportunity to incorporate flow cytometry and immune monitoring data in basic, pre-clinical and clinical cancer-related research.
FCCS services are broken into two categories: flow cytometry and immune monitoring.
Flow cytometry services include multi-parameter cell phenotyping (membrane, cytoplasmic and nuclear antigens); DNA content, cell cycle analysis and apoptosis analysis; cell cytotoxicity assays; intracellular cytokine and protein analysis; signal transduction and cell activation (e.g., calcium flux, pH, oxidative activity); membrane fluidity analysis; 4-way sorting under BSL-2 containment; and single cell cloning in 12, 24, 96 and 384 well microplates.
Immune monitoring services were newly expanded and implemented in June 2017. Cell services include: isolation of immune cells from human blood and tissues; generation of dendritic cells and macrophages from blood and/or tumor tissue; expansion of T cells from blood and/or tumor tissue; and analysis of cytokines, growth factors, other soluble factors via ELISPOT, ELISA and Luminex xMAP system. Immune functional assays include: antigen-specific T cell proliferation, cytokine production and antibody synthesis; and cytotoxicity assays for CD8 T cells and NK cells.
Researchers can schedule to have their samples analyzed or sorted for membrane, cytoplasmic and nuclear antigen expression, light-scattering properties, DNA content, cell cycles, and apoptosis, as well as measured for intracellular biochemical changes such as calcium flux and pH. Researchers must schedule time at least 72 hours in advance. For more information, visit the Flow Cytometry and Cell Sorting website.
Redox Metabolism Shared Resource Facility
The Redox Metabolism Shared Resource Facility (RM SRF) was established to provide expertise in and analyses of free radicals, cellular energetics, and reactive species, as well as proteomics in cancer and cancer biology for Markey Cancer Center investigators who perform basic, pre-clinical, and clinical research. For more information, visit the RM SRF website.