Instructions for submitting RNA samples for NanoString
Submit cellular total RNA extracts ( ≥100 ng in 5ul RNase-free H2O or buffer) or cellular lysate of 10,000 cells in ≥ 5ul lysis buffer (e.g. RLT), in sterile microfuge tubes.
*For miRNA assays, submit ≥165 ng cellular RNA in 5ul RNase-free H2O.
Preparation of RNA
The core facility staff does not extract and prepare RNA from cells or tissues.
Note: The quality of the RNA is essential to the overall success of the GeneChip analysis. Since the most appropriate protocol for the isolation of RNA can be source material-dependent, we recommend using a protocol that has been established for the tissues and cells being used. Or if you do not have
an established protocol, we suggest using one of the commercially available kits designed for RNA isolation, following the manufacturer's instructions.
Good quality RNA has been obtained using the kits listed below:
- Total RNA preparation from cells: QIAGEN's RNeasy Total RNA Isolation kit.
- Total RNA preparation from tissues: Invitrogen Life Technologies' TRIzol reagent.
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