Next Generation Sequencing (NGS)
Next-generation sequencing, also known as massively parallel sequencing, is the term used to describe the modern sequencing technology. Next-generation sequencing is capable to sequencing millions of copies of DNA fragments in a single reaction.
UK HealthCare Genomics Core Laboratory has three Illumina® next-generation sequencing systems, one HiSeq 2500 and two MiSeq. The Illumina next-generation sequencing systems use sequencing by synthesis (SBS) technology and support both single-read and paired-end libraries.
Illumina HiSeq 2500
The HiSeq 2500 is the most widely adopted system worldwide and generates a massive amount of sequence on every run. This system features two run modes, rapid run and high output run, and has the ability to process one or two flow cells simultaneously.
The rapid run mode, using two lane flow cell, provides shorter turnaround. It is most suitable for small scale projects, such as sequencing of a few whole exomes, whole transcriptomes, small genomes, etc. The rapid run supports the longer paired-end reads (250 bp), which is important for de novo assembly and also in metagenomic analysis. The rapid mode can generate up to 600 million paired-end reads on a single flow cell and up to 1.2 billion reads on dual flow cell. The high output mode uses eight lane flow cell and produces more than 4 billion paired-end reads on single flow cells and more than 8 billion paired-end reads on dual flow cells. It is well suited for larger studies or studies that require greater depth of coverage.
The Illumina MiSeq instrument is a desktop sequencer that is capable of generating up to 25 million (v2 kits) – 45 million (v3 kits) raw paired-end reads per run within a day. MiSeq supports the paired-end 300 bp reads (2X300). It is most suitable for amplicon sequencing, targeted resequencing, small genomes, etc.
The following NGS services are currently offered:
- Whole genome sequencing
- Amplicon sequencing
- 16S sequencing
- Targeted resequencing
- Whole exome sequencing
- Whole transcriptome sequencing (RNA-Seq)
- Targeted RNA-Seq
- Sequencing of DNA fragments captured by chromatin immunoprecipitation assay (ChIP-Seq)
- Whole genome methylation detection (Methylation-Seq)
In addition, Genomics Core Laboratory also offers sequencing library preparation services.
HiSeq pricing »
MiSeq pricing »
NGS library preparation pricing »
Sample submission instructions »