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Next Generation Sequencing

Next-generation sequencing, also known as massively parallel sequencing, is the term used to describe the modern sequencing technology. Next-generation sequencing is capable to sequencing millions of copies of DNA fragments in a single reaction. 

UK HealthCare Genomics Core Laboratory has three Illumina® next-generation sequencing systems, one HiSeq 2500 and two MiSeq. The Illumina next-generation sequencing systems use sequencing by synthesis (SBS) technology and support both single-read and paired-end libraries.

Illumina HiSeq 2500

The HiSeq 2500 is the most widely adopted system worldwide and generates a massive amount of sequence on every run. This system features two run modes, rapid run and high output run, and has the ability to process one or two flow cells simultaneously.

The rapid run mode, using two lane flow cell, provides shorter turnaround. It is most suitable for small scale projects, such as sequencing of a few whole exomes, whole transcriptomes, small genomes, etc. The rapid run supports the longer paired-end reads (250 bp), which is important for de novo assembly and also in metagenomic analysis.  The rapid mode can generate up to 600 million paired-end reads on a single flow cell and up to 1.2 billion reads on dual flow cell. The high output mode uses eight lane flow cell and produces more than 4 billion paired-end reads on single flow cells and more than 8 billion paired-end reads on dual flow cells. It is well suited for larger studies or studies that require greater depth of coverage.

Illumina MiSeq

The Illumina MiSeq instrument is a desktop sequencer that is capable of generating up to 25 million (v2 kits) – 45 million (v3 kits) raw paired-end reads per run within a day. MiSeq supports the paired-end 300 bp reads (2X300). It is most suitable for amplicon sequencing, targeted resequencing, small genomes, etc.

The following NGS services are currently offered:

  • Whole genome sequencing
  • Amplicon sequencing
  • 16S sequencing
  • Targeted resequencing
  • Whole exome sequencing
  • Whole transcriptome sequencing (RNA-Seq)
  • Targeted RNA-Seq
  • Sequencing of DNA fragments captured by chromatin immunoprecipitation assay (ChIP-Seq)
  • Whole genome methylation detection (Methylation-Seq) 

In addition, Genomics Core Laboratory offers sequencing library preparation services.

  • HiSeq Pricing

    Name of service, description, price (Note: Non-UK clients pay list price plus 10 percent):

    High Output Run Mode

    RES HOP FULL FLOW CELL SR 100, HiSeq high output run with a 100 bp single read full flow cell, $9,200.00

    RES HOP 1 LANE SR 100, HiSeq high output run with a 100 bp single read 1 lane, $1,150.00

    RES HOP FULL FLOW CELL PE 50, HiSeq high output run with a 50 bp paired end read full flow cell, $11,261.00

    RES HOP 1 LANE PE 50, HiSeq high output run with a 50 bp paired end read 1 lane, $1,493.96

    RES HOP FULL FLOW CELL PE 100, HiSeq high output run with a 100 bp paired end read rull rlow cell, $16,396.26

    RES HOP 1 LANE PE 100, HiSeq high output run with a 100 bp paired end read 1 lane, $2,107.46

    RES HOP FULL FLOW CELL PE 125, HiSeq high output run with a 125 bp paired end read full flow cell, $16,396.26

    RES HOP 1 LANE PE 125, HiSeq high output run with a 125 bp paired end read 1 lane, $2,107.46

    RES HOP FULL FLOW CELL SR 50, HiSeq high output run with a 50 bp single read full flow cell, $7,000.00

    RES HOP 1 LANE SR 50, HiSeq high output run with a 50 bp single read 1 lane, $971.96

    Rapid Run Mode

    RES HISEQ RR FULL FLOW CELL SR 50, HiSeq rapid run with a 50 bp single read full flow cell, $1,650.00

    RES SPLIT FLOW CELL 2 LANES SR50, HiSeq rapid run with a 50 bp single read 2 lanes split flow cell, $2,300.00

    RES SPLIT FLOW CELL 1 LANE SR50, HiSeq rapid run with a 50 bp single read 1 lane split flow cell, $1,150.00

    RES HISEQ RR FULL FLOW CELL SR 100, HiSeq rapid run with a 100 bp single read full flow cell, $2,300.00

    RES SPLIT FLOW CELL 2 LANES SR100, HiSeq rapid run with a 100 bp single read 2 lanes split flow cell, $2,700.00

    RES SPLIT FLOW CELL 1 LANE SR100, HiSeq rapid run with a 100 bp single read 1 lane split flow cell, $1,350.00

    RES HiSEQ RR FULL FLOW CELL SR 200, HiSeq rapid run with a 200 bp single read full flow cell, $3,063.00

    RES SPLIT FLOW CELL 2 LANES SR200, HiSeq rapid run with a 200 bp single read 2 lanes split flow cell, $3,549.00

    RES SPLIT FLOW CELL 1 LANE SR200, HiSeq rapid run with a 200 bp single read 1 lane split flow cell, $1,775.00

    RES HISEQ RR FULL FLOW CELL PE50, HiSeq rapid run with a 50 bp paired end read length in full flow cell, $3,115.00

    RES SPLIT FLOW CELL 2 LANES PE50, HiSeq rapid run with a 50 bp paired end read 2 lanes split flow cell, $3,601.00

    RES SPLIT FLOW CELL 1 LANE PE50, HiSeq rapid run with a 50 bp raired end read length in 1 lane split flow cell, $1,801.00

    RES HISEQ RR FULL FLOW CELL PE100, HiSeq rapid run with a 100 bp paired end read full flow cell, $4,009.00

    RES SPLIT FLOW CELL 2 LANES PE100, HiSeq rapid run with a 100 bp paired end read 2 lanes split flow cell, $4,495.00

    RES SPLIT FLOW CELL 1 LANE PE100, HiSeq rapid run with a 100 bp paired end read 1 lane split flow cell, $2,300.00

    RES HISEQ RR FULL FLOW CELL PE250, HiSeq rapid run with a 250 bp paired end read full flow cell, $6,364.30

    RES SPLIT FLOW CELL 2 LANES PE250, HiSeq rapid run with a 250 bp paired end read 2 lanes split flow cell, $6,960.23

    RES SPLIT FLOW CELL1 LANE PE250, HiSeq rapid run with a 250 bp paired end read 1 lane split flow cell, $3,513.22

  • MiSeq Pricing

    Name of service, description, price (Note: Non-UK clients pay list price plus 10 percent):

    RES MISEQ RUN 2X250 V2 NANO, MiSeq run - Nano 250X 2 flow cell. Run only, QPCR may be required before run. $900.00

    RES MISEQ RUN 2X150 V2, MiSeq run -150X2 flow cell. Run pnly,QPCR may be required before run.$1,205.03

    RES MISEQ RUN 2X250 V2, MiSeq run - 250X2 flow cell. Run only, QPCR may be required before run. $1,285.11

    RES MISEQ RUN 2X300 V3, MiSeq run - 300X2 flow cell. Run only, QPCR may be required before run.$1,664.71

  • NGS Library Preparation Pricing

    Name of service, description, price (Note: Non-UK clients pay list price plus 10 percent):

    RES Q PCR FOR NGS LIBRARY, QPCR for NGS, $35.00

    RES NEXTERA LIBRARY PREP**, MiSeq Nextera library preparation (client provides DNA), $250.00

    RES NEXTERA XT LIBRARY PREP**, MiSeq Nextera XT library preparation (client provides DNA), $256.00

    RES TRUSEQ PCR-FREE LIBRARY PREP**, MiSeq TruSeq DNA PCR free library preparation, $255.00

    RES TRUSEQ NANO LIBRARY PREP**, MiSeq TruSeq DNA library preparation, $325.00

    RES TRUSEQ RNA LIBRARY PREP**, MiSeq TruSeq RNA library preparation, $310.79

    ** Library Preparation includes ONLY Bio-Analysis and Qubit Cost and NOT the QPCR cost of $35.